TABLE 1.
Strains and plasmids used in this study.
| Strain/plasmid | Description | Sources |
| Strains | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| BY4742 | MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0 | Su et al., 2020 |
| BL03-D-4 | BY4742, ΔGal80:: PHSP26-CrtB-TADH1-PHSP26-CrtI-TGPM1-PHSP26-CrtE-TCYC1,Δ416d PCit1-tHMGR-TGuo, ΔAld6 | Unpublished |
| SC101 | BL03-D-4, ΔPxk:: PEFT1-XYL1-Tsynth7-PPGK1-XYL2-Tguo1-PHSP82 | This study |
| SC102 | BL03-D-4, ΔPxk:: PEFT1-XYL1m-Tsynth7-PPGK1-XYL2-Tguo1-PHSP82 | This study |
| SC103 | SC102,Δ720a:: PHSP104-xPk-Tguo1-PSSA1-PTA-Tsynth6 | This study |
| SC104 | SC103,ΔPho13 | This study |
| SC105 | SC104,ΔPGal2::PSSA1-Gal2m | This study |
| SC106 | SC105,Δ911b :: His3-Leu2-Ura3 | This study |
| Plasmids | ||
| pHCas9-gRNA | pBR322-derived vector, including HCas9, KanMX, gRNA and 2 μ ori, ampicillin resistant | Su et al., 2020 |
| pHCas9-XK | pHCas9-gRNA targeted to the XK loci | This study |
| pHCas9-Pho13 | pHCas9-gRNA targeted to the Pho13 loci | This study |
| pHCas9-Gal2 | pHCas9-gRNA targeted to the Gal2 loci | This study |
| pHCas9-720a | pHCas9-gRNA targeted to the 720a loci | Su et al., 2020 |
| pHCas9-911b | pHCas9-gRNA targeted to the 911b loci | Su et al., 2020 |
| pACYC-XK | Plasmid including the modules of PEFT1-XYL1-Tsynth7-PPGK1-XYL2-Tguo1-PHSP82 | This study |
| pACYC-PK | Plasmid including the modules of PHSP104-xPk-Tguo1-PSSA1-PTA-Tsynth6 | This study |
| pACYC-pro | Plasmid including the modules of His3-Leu2-Ura3 | Su et al., 2020 |