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. 2020 May 14;8:338. doi: 10.3389/fcell.2020.00338

FIGURE 2.

FIGURE 2

Conditional knockout of Dicer within hair follicle bulge stem cells. (A) Images of the depilated regions of control (Dicerflox/flox;K15PR1Cre–) and experimental (Dicerflox/flox;K15PR1Cre+) mice at 0 days post depilation (DPD) and 9DPD. (B) Histological analysis of 9DPD skins in both control (Dicerflox/flox;K15PR1Cre–) and experimental (Dicerflox/flox;K15PR1Cre+) mice. Boxed region is magnified in the lower panels. Thickness measured from the panniculus carnosus (pc; muscle) to the outer epidermal layer (e). Bars (top panels = 1 mm; bottom panels = 100 um). (C) Hair follicle count and skin thickness analysis. Serial skin sections were assessed from control and Dicer cKO mice (n = 12; p ≤ 0.001, Student’s t test). (D) Whole-mount uDISCO analysis of 9DPD skins in both control (Dicerflox/flox;K15PR1Cre–) and experimental (Dicerflox/flox;K15PR1Cre+) mice. (E) Schema of the targeting construct for generation of Dicer floxed mice. (F) Dicer deletion PCR using RU486 pre- and post-treated tissue samples. Refer to E, schema of the PCR strategy to determine Dicer ablation efficiency within tissue sample.