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Kinetic analysis of the binding of M. bovis recombinant MilA to the fluorescent substrate 1,8-ANS
| Protein construct | Vmax (min−1)b | Km (μM) | R-squared value | Efficiency (min−1 · μM−1)a |
|---|---|---|---|---|
| MilA-ab | 41,121 | 54.61 | 0.9967 | 752.9 |
| MilA-CD | 130,802 | 253.6 | 0.9949 | 515.7 |
| MilA-EF | 61,909 | 53.11 | 0.9977 | 1,165.6 |
Efficiency = Vmax/Km.
Vmax, maximum rate of metabolism.
