FIG 2.

Induction of partly mitochondrial apoptosis by P. acanthamoebae infection in macrophages. HoxB8 macrophages were infected with P. acanthamoebae for 24 or 48 h. (A) HoxB8 macrophages (wild type [WT] or Bax/Bak deficient) were infected for 24 h and analyzed by flow cytometry to detect active caspase-3. (B) HoxB8 macrophages (WT or Bax/Bak deficient) were infected for 24 or 48 h and analyzed by flow cytometry to detect cell death by live/dead staining. The MOI was 2.5. p.c., positive control, treatment with Mcl-1-inhibitor S63845 (3 μM) and ABT-737 (5 μM) for 4 h; h.-t., heat treated (1 min at 95°C). Experiments for positive controls were done separately. Data are shown as means/SEM of at least three independent experiments *, P < 0.05 (unpaired two-tailed t test) between control and mutant cells.