FIG 5.

Generation of infectious progeny of P. acanthamoebae in Bax/Bak-deficient HeLa cells. HeLa CTRL or Bax/Bak-deficient cells were infected with P. acanthamoebae. After 24 h or 48 h, cultures were mechanically disrupted, and the suspension was used to infect amoeba (Neff). Twenty-four hours after amoebal infection, cultures were lysed, DNA was extracted, and P. acanthamoebae DNA was quantified by qPCR. For comparison, A. castellanii was infected with an MOI of 1 for 24 h. P. acanthamoebae genomic DNA per 50 ng of total DNA isolate is shown. Data are means/SEM of at least three independent experiments. *, P < 0.05 (paired two-tailed t test).