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cagA copy number and IL-8 induction in mouse-passaged H. pylori. cagA copy number was determined by real-time PCR. Those mouse-passaged isolates with cagA copy number outside two standard deviations of the mean of all wild-type controls run on each assay were defined as changed. Most isolates showed either an increase or decrease in the copy number of cagA (A), and cagA was deleted in three cases, but copy number was not correlated with level of IL-8 induction (B).
