FIG 1.
CBU2072 is essential for intracellular replication of C. burnetii. (A and B) Intracellular replication of C. burnetii WT (red), 2072::Tn mutant (blue), 2072::Tn pFLAG-2072 mutant (green), and 2072::Tn pFLAG-207224–165 mutant (purple) in HeLa CCL2 cells (n = 4) (A) and THP1 cells (n = 5) (B). THP1 cell data depict fold changes at 3 days postinfection. Error bars represent standard deviation. *, P < 0.05. P values were determined using an unpaired Student t test. (C) Representative confocal immunofluorescence (IF) images at 3 days postinfection for HeLa CCL2 and THP1 cells. Cells were stained with anti-LAMP1 (green), anti-C. burnetii (red), and DAPI (blue). Scale bar = 10 μm. Arrows indicate individual intracellular C. burnetii cells.