FIG 6.

Absence of EirA leads to an accumulation of some amino acids and significantly lower glycolytic and TCA cycle activities. Shown are combined metabolites detected by GC-MS and LC-MS which were significantly different in abundance between the C. burnetii eirA::Tn mutant and WT strains. Red denotes metabolites which were significantly higher in abundance in the C. burnetii eirA::Tn mutant, and blue denotes metabolites which were significantly lower in abundance in the C. burnetii eirA::Tn mutant. P < 0.05, BH-adjusted unpaired t test. Pale blue denotes metabolites which were not significant. Pale green denotes enzymes which are missing based on genome annotation data in KEGG. Purple denotes putative and defined metabolite transporters. Dotted arrows indicate pathways which have been abbreviated. Metabolite abbreviations are listed in Table S1 in the supplemental material.