FIG 7.
Absence of EirA does not impact susceptibility to cell membrane stressors or effect overall gross morphology in C. burnetii. C. burnetii WT and eirA::Tn, eirA::Tn pFLAG-EirA, and eirA::Tn pFLAG-EirA24–165 mutant strains were exposed to various concentrations of either ampicillin or polymyxin B at 4 days postinoculation, and the impact on bacterial replication was quantified at 24 h posttreatment by genome equivalents (GE) (A) and CFU (B) per milliliter. Fold change depicts the bacterial numbers at 24 h posttreatment relative to the numbers pretreatment. No significant differences in GE and CFU were observed based on an unpaired Student t test. (C) C. burnetii WT, eirA::Tn mutant, and eirA::Tn pFLAG-EirA mutant strains were grown for 6 days in ACCM-2 before being imaged using transmission electron microscopy (TEM). Images are representative of two technical replicates.