TABLE 3.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ to 3′) | Comments and/or referencea |
|---|---|---|
| eirA_F1 | AAAGGATCCATGCGTTATCCGAAATTCAGT | Used to amplify eirA to clone into 3′ end of 3×FLAG tag in pJB-Kan:3×FLAG-MCS |
| eirA_F2 | ATAAGCTTCTGCAGATGCGTTATCCGAAATTCAGTT | Used to amplify eirA to clone into 5′ end of 3×FLAG tag in pJB-Kan:3×FLAG |
| eirA24–165_F | AAAGGATCCGCCCCTGCAACG | Used to amplify eirA24–165 to clone into 3′ end of 3×FLAG tag in pJB-Kan:3×FLAG-MCS |
| eirA_R1 | AAGCGGCCGCTTAAAATCCAATTTCTTGTTG | Used to amplify eirA and eirA24–165 to clone into 3′ end of 3×FLAG tag in pJB-Kan:3×FLAG-MCS |
| eirA_R2 | ATGTCGACCTACTTGTCATCGTCATCCTT | Used to amplify eirA to clone into 5′ end of 3×FLAG tag in pJB-Kan:3×FLAG |
| mceA_F | AAGGTCGACATGAGACAACTCGTTTCAATTAAA | 32 |
| mceA_R | AAGGTCGACTTACATAATAGAACACCCACGA | 32 |
| cbu0425_F | AAGGATCCATGGAAGGCGTAATCTATTCA | Used to amplify cbu0425 to clone into pJB-Kan:BlaM-MCS (8) |
| cbu0425_R | AACTGCAGTTATGTGGATGCGCTGGGC | Used to amplify cbu0425 to clone into pJB-Kan:BlaM-MCS (8) |
| ompA_F | CAGAGCCGGGAGTCAAGCT | 44 |
| ompA_R | CTGAGTAGGAGATTTGAATCGC | 44 |
a
MCS, multiple-cloning site.