TABLE 4.
Plasmids used in this study
| Plasmid | Propertiesa | Reference or source |
|---|---|---|
| pJB-Kan:BlaM | C. burnetii expression vector encoding BlaM from Bacillus subtilis under constitutive expression by the cbu1169 promoter (P1169), Kanr | Heinzen Laboratory |
| pJB-Kan:BlaM-MCS | C. burnetii expression vector encoding BlaM from B. subtilis and MCS from pcDNA4/TO under constitutive expression by the P1169 promoter, Kanr | This study |
| pJB-Kan:BlaM-0425 | Encodes 5′-BlaM tag and cbu0425, Kanr | This study |
| pJB-Kan:BlaM-MceA | Encodes 5′-BlaM tag and mceA or cbu0077, Kanr | Newton Laboratory |
| pJB-Kan:3×FLAG | C. burnetii expression vector encoding 5′-3×FLAG tag under constitutive expression by the C. burnetii P1169 promoter, Kanr | 48 |
| pJB-Kan:3×FLAG-MCS | C. burnetii expression vector encoding 5′-3×FLAG tag and MCS from pcDNA4/TO under constitutive expression by the C. burnetii P1169 promoter, Kanr | This study |
| pJB-Kan:3×FLAG-MceA | Encodes 5′-BlaM tag and mceA or cbu0077, Kanr | Newton Laboratory |
| pJB-Kan:3×FLAG-EirA | pJB-Kan:3×FLAG encoding 5′-3×FLAG tag and eirA, Kanr | This study |
| pJB-Kan:EirA-3×FLAG | pJB-Kan:3×FLAG encoding 3′-3×FLAG-tagged eirA, Kanr | This study |
| pJB-Kan:3×FLAG-EirA24–165 | pJB-Kan:3×FLAG encoding 5′-3×FLAG tag and truncated eirA from bp 70–498 (amino acid residues 24–165), Kanr | This study |
| pMAL-c2X:EirA24–165 | pMAL-c2X encoding 5′-MBP tag and truncated eirA from bp 70–498 (amino acid residues 24–165), Ampr | This study |
a
Kanr, kanamycin resistance; Ampr, ampicillin resistance.