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. 2020 May 20;88(6):e00100-20. doi: 10.1128/IAI.00100-20

FIG 5.

FIG 5

The effect of aar and aggR on gene expression in DH5α transformed with paar and/or paggR/paggR-D. (A) DH5α was transformed with paar and paggR expressing full-length aggR or their corresponding empty vectors pBR322 and pACYC177, respectively. Transcriptional levels of E. coli chromosomal genes orf1228 and orf2223 were analyzed by qRT-PCR. (B) DH5α was transformed with paar and paggR-D expressing the AggR dimerization domain or their corresponding empty vectors. Transcriptional levels of E. coli chromosomal genes orf1228 and orf2223 were analyzed by qRT-PCR. RT-PCR data are representative of at least three independent experiments. Asterisks indicate significant differences by ANOVA (*, P < 0.05; **, P < 0.005; ***, P < 0.0005).