FIG 6.

The effect of aar on gene expression in the presence or absence of hns in DH5α. (A) DH5α and DH5αΔhns were transformed with paar or its corresponding empty vector pBR322. Transcriptional levels of ompX were analyzed by qRT-PCR. (B) DH5αΔhns was transformed with paar and phns or their corresponding empty vectors pBR322 and pKNT25, respectively. Transcriptional levels of orf1228 were analyzed by qRT-PCR. RT-PCR data are representative of at least three independent experiments. Asterisks indicate significant differences by ANOVA (*, P < 0.05; **, P < 0.005; ***, P < 0.0005).