FIGURE 7.

Analysis on gel electrophoresis of the activity of the different superoxide dismutase (SOD) isozymes of red pepper fruit samples preincubated with different chemicals. (A) Samples were preincubated with 4 mM of SIN-1, 4 mM of GSNO, 4 mM of DEA NONOate, and 4 mM of GSH; 10 mM of H₂O₂; and 10 mM of DTT. After electrophoresis (acrylamide 8%), gels were stained for SOD activity. (B) Samples were processed as in panel (A), but the gel was preincubated with 5 mM of H₂O₂ before staining for SOD activity. (C) Samples were processed as in panel (A), but the gel was preincubated with 5 mM of GSH prior to SOD activity staining. The gel pictures are representative of those obtained from at least three biological replicas repeated three times.