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. 2020 May 1;23(5):101114. doi: 10.1016/j.isci.2020.101114

Figure 1.

Figure 1

In the PVN of Male and Female Sapphire Mice with Diet-Induced Obesity (DIO), There Is Neuronal Loss, but the Number of Sim1/MC4R Neurons Is Instead Unchanged

(A–H) Confocal microscopy of brain sections from male and female Sapphire mice exposed to LF diet (LFD) and HFD (HFD). Males, LFD, n = 6–7 and HFD, n = 6–7; females, LFD, n = 6–7 and HFD, n = 6–7. (A) and (E) Intensity of α-MSH immunostaining in arcuate nucleus derived from four maximum intensity projection (MIP) images per mouse. (B) and (F) Number of NeuN-expressing cells and number of GFP-expressing Sim1/MC4R neurons in the PVN derived from two MIP images per mouse. (C) and (G) Number of BrdU-positive nuclei in PVN derived from two MIP images per mouse. (D) and (H) Percentage of PVN Sim1/MC4R neurons with BrdU-positive nuclei from two MIP images per mouse. Dotted line in (A) and (E) outlines arcuate nucleus; dotted line in (B), (C), (F) and (G) outlines PVN; scale bars in (A–C) and (E–G), 60 μm; scale bars in (D) and (H), 10 μm; data in all graphs are normalized. ∗∗p < 0.01, ∗∗∗p < 0.001; ∗∗∗∗, p < 0.0001. Data are represented as mean values +/- SD.