Figure 2.

In Sim1/MC4R Neurons of Male and Female Mice, DIO Induces Loss of CoxIV Abundance, Decreased Mitochondria Content, and Extension of Mitochondrial Network

(A–D) Fluorescence microscopy of brain sections from Sapphire mice exposed to LF diet (LFD) and HF diet (HFD). (A) and (C) Intensity of CoxIV immunostaining (red fluorescence) in Sim1/MC4R neurons identified by GFP immunostaining (green fluorescence) in PVN of males (LFD, n = 5 and HFD, n = 5) and females (LFD, n = 8, HFD, n = 7). Each data point was obtained by taking the average of CoxIV intensity from 25 to 30 Sim1/MC4R neurons using five confocal images taken with 60× objective per mouse. Data are normalized; scale bar, 20 μm. (B) and (D) Super-resolution fluorescence microscopy images of sections from male Sapphire mice (LFD, n = 5 and HFD, n = 5) and female Sapphire mice (LFD n = 5 and HFD n = 5). Mitochondria size is in μm²; mitochondrial coverage area is normalized; scale bar, 5 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data are represented as mean values +/- SD.