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. 2020 May 1;23(5):101114. doi: 10.1016/j.isci.2020.101114

Figure 3.

Figure 3

Exposure to Elevated Palmitate Is Sufficient to Induce Loss of CoxIV and to Disrupt Mitochondrial Function in Primary Hypothalamic Neurons

(A–D) Confocal microscopy of Neuro2A cells stably expressing HA-MC4R-GFP and incubated with and without palmitate for 16 h. HA-MC4R-GFP (green), KDEL immunostaining (red), and COXIV immunostaining (magenta). (B–D) Quantification of HA-MCR-GFP fluorescence, KDEL and COXIV immunostaining. Each symbol in graph corresponds to the value measured from one cell (n = 75 cells).

(E–G) Confocal microscopy of primary hypothalamic neurons treated without palmitate or with 250 μM palmitate for 48 h and exposed to MitoTracker for 30 min, fixed and immunostained for CoxIV. (F) Quantification of MitoTracker fluorescence intensity by live microscopy. (G) Quantification of CoxIV immunostaining in images of fixed cells including that in (F). Data from 12 to 13 cells per condition are normalized; scale bars, 10 μm. ∗∗p < 0.01. ∗∗∗∗ p < 0.0001. Data are represented as mean values +/- SD.