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. 2020 May 1;23(5):101114. doi: 10.1016/j.isci.2020.101114

Figure 7.

Figure 7

In the PVN of Male and Female MC4R-HA+/- Mice Returned to Normal Weight After DIO, Neurogenesis Is Restored

(A–D) Confocal microscopy of PVN sections of male and female MC4R-HA+/- mice treated with LFD, HFD, and HFD to LFD. (A and C) PVN sections are immunostained with anti-BrdU and co-stained with DAPI, males LFD, n = 8; HFD, n = 7; HFD to LFD n = 8; females, LFD, n = 6; HFD, n = 7; HFD to LFD n = 7; number of cells with nuclear BrdU is derived from two MIP images per mouse; scale bars: upper, 60 μm; lower, 8 μm. (B and D) PVN sections are immunostained with anti-BrdU and anti-DCX, males LFD, n = 8; HFD, n = 8; HFD to LFD n = 8; females, LFD, n = 7; HFD, n = 6; HFD to LFD n = 8; number of DCX and nuclear BrdU-positive cells are derived from eight confocal images per mouse taken along the third ventricle (3V) with the 60× objective; scale bars: upper, 30 μm; lower, 16 μm. ∗p < 0.05, ∗∗p < 0.01. Data are represented as mean values +/- SEM.