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. 2020 May 1;23(5):101114. doi: 10.1016/j.isci.2020.101114

Figure 8.

Figure 8

In Male and Female MC4R-HA+/- Mice Returned to Normal Weight after DIO, Expression of MC4R in Sim1/MC4R Neurons Is Recovered, Whereas Loss of Other PVN Neurons Persists

(A) Arcuate nucleus sections of male mice treated with LFD (n = 7), HFD (n = 6), and HFD to LFD (n = 7) are immunostained with anti-α-MSH.

(B–E) Confocal microscopy of PVN sections of male and female MC4R-HA+/- mice treated with LFD, HFD, and HFD to LFD. (B and D) PVN sections are immunostained with anti-NeuN and co-stained with DAPI, males LFD, n = 5; HFD, n = 6; HFD to LFD n = 6; females, LFD, n = 6; HFD, n = 6; HFD to LFD n = 6. (C and E) PVN sections are immunostained with anti-HA and co-stained with DAPI, males LFD, n = 5; HFD, n = 6; HFD to LFD n = 6; females, LFD, n = 6; HFD, n = 6; HFD to LFD n = 6. Data in graphs are normalized and derived from two MIP images per mouse. Scale bars, 60 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ∗∗∗∗, p < 0.0001. Data are represented as mean values +/- SEM.