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. 2020 May 19;11(3):e00135-20. doi: 10.1128/mBio.00135-20

FIG 6.

FIG 6

The fusion activity of WT, H144A, and Q258A JEVs. (A) DiO/R18 double-labeled viruses were allowed to infect Vero cells for different times. Bar, 10 μm. (B) Mean fluorescence intensities (MFIs) of DiO in cells infected by the double-labeled viruses for different times measured by flow cytometry (n = 3). (C) MFIs of DiO in cells treated with drugs and infected by JEV for 1 h. NH4Cl and chloroquine (CQ) were used to block virus-endosome fusion (n = 3).