Comparison of the Vps4 (surface representation, gray) in complex with ESCRT-III subunits (ribbon representation, blue) in Asgard archaea. The MIM1 and MIM2 are shown in orange (stick representation, orange) and highlighted in red in close-up views (space-filling representation). The black letters indicate the main residues in the MIT domains that contribute to the interaction. The Vps4 MIT domain in complex with Vps2/24/46 (A) and Vps20/32/60 (B) subunits in S. cerevisiae, Heimdallarchaeota_LC_3 (Heimdall_LC_3), Odinarchaeota_LCB_4 (Odin_LCB_4), Thorarchaeota_AB_25 (Thor_AB_25), and Lokiarchaeum_GC14_75 (Loki_GC14_75) are indicated.