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. 2020 May 19;11(3):e00920-20. doi: 10.1128/mBio.00920-20

FIG 3.

FIG 3

Role of FA kinase in S. aureus PG molecular species composition in the infected thigh. The thigh lipid samples were separated by solid-phase extraction, and the PG molecular species containing 15:0 were detected using mass spectrometry by scanning for parent ions that lose m/z 241 corresponding to the loss of a 15:0 FA. The identities of the FAs in the 1 and 2 positions (blue) are shown in panels A and B for each molecular species (black). (A) Wild-type strain AH1263 grown in rich laboratory medium. (B) Strain AH1263 PG recovered from an infected thigh. (C) Strain JLB2 (ΔfakA) PG recovered from an infected thigh. (D) Strain JLB31 (ΔfakB1 ΔfakB2) PG recovered from an infected thigh. (E) Strain JLB27 (ΔfakB1) PG recovered from an infected thigh. (F) Strain JLB30 (ΔfakB2) PG recovered from an infected thigh. Representative spectra are shown, and the triplicate results are tabulated in Table S1 in the supplemental material. The peak labeled “X” is not a PG molecular species.