TABLE 3.
Serological profile of strain Cam853 and other related serotypes by flow cytometrya
| Strain | Antiserum |
|||||||
|---|---|---|---|---|---|---|---|---|
| Pool E | Pool S | Type 39 | Group 10 | FS10b | FS10d | FS10f | FS6d | |
| Cam853 | + | + | + | − | − | + | − | − |
| SSISP39 | + | − | + | − | − | + | − | − |
| SSISP10A | + | + | + | + | − | + | − | − |
| SSISP10B | + | + | − | + | + | + | − | − |
| SSISP10C | + | + | − | + | + | − | + | − |
| SSISP10F | + | + | − | + | + | − | − | − |
| MJC705 | − | − | − | − | − | − | − | + |
Pneumococcal strains SSISP10A, SSISP10B, SSISP10C, SSISP10F, and SSISP39 representing serotypes 10A, 10B, 10C, 10F, and 39, respectively, were procured from Statens Serum Institut (SSI), Copenhagen, Denmark. MJC705 strain represents the serotype 6C and was obtained from the Nahm laboratory bacterial strain collection. Cam853 is the 10D cps-containing strain and was obtained from during a post-PCV13 introduction colonization study at Angkor Hospital for Children/Cambodia Oxford Medical Research Unit. Pool E and pool S were designed to react with multiple serotypes (58). Group serum reacts to all the serotypes within one serogroup. Type serum reacts only with a single serotype. Factor serum (FS) reacts with a specific epitope in a serotype. All the antisera are polyclonal rabbit antisera and were obtained from SSI. Negative reactions (−) and positive reactions (+) are shown. Cam853 reacted only with FS10d in agglutination reaction and flow cytometry. Cam853 also reacted with pool E and S antisera in flow cytometry, which otherwise showed negative results in agglutination reaction.