Figure 3.

Relative mRNA Expressions and In Vitro Activities of Enzymes Involved in Glycogen Metabolism Are Selectively Reduced at 12 h after OGD/R in Cultured Astrocytes

(A–F) The mRNA levels of GS (A), GBE1 (B), PYGB (C), PYGM (D), PYGL (E), and AGL (F) were determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR) at 12 h after reoxygenation in cultured astrocytes (n = 8, independent t test).

(G–L) Quantified GS (G), GP (H), PhK (I), PKA (J), GSK3α (K), and GSK3β (L) activities in astrocytes at 12 h after reoxygenation in cultured astrocytes (n = 8, independent t test).

The data are presented as the mean ± SEM. ^∗∗∗p < 0.001. See also Figure S2 and Table S3.