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. 2020 Mar 5;10(1):171–190. doi: 10.1016/j.jcmgh.2020.02.006

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Necrox protects HIOs from Stx-induced necrosis. (A) HIOs were microinjected with Stx2a (30 ng) and Necrox 5 (2 μmol/L) and incubated for 27 hours. Cryosections were stained for necrosis (Sytox-Green). Scale bar: 100 μm. Representative images of experiments performed in triplicate are shown. (B) Cellular death by necrosis was quantified by fluorescence using ImageJ software, and plotted as means ± SD, n = 3. Statistical significance compared with saline control was assessed using 1-way analysis of variance, Bonferroni posttest; ∗∗∗P < .0001. DAPI, 4′,6-diamidino-2-phenylindole; L, lumen; M, mesenchyme.