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. 2020 Mar 5;10(1):171–190. doi: 10.1016/j.jcmgh.2020.02.006

Figure 6.

Figure 6

Mesenchymal–epithelial transition is induced by other bacterial toxins. (A) Isolated mesenchymal cells derived from mRuby2 E-cadherin HIOs were challenged by addition of LPS (10 ng), microcystin-LR (10 ng), TcdA (100 ng), or TcdB (100 ng) in 500 μL gut media. Scale bar: 100 μm. Left: fluorescent (mRuby2) image shown alone. Right: merged with bright-field (BF) image. Representative images of experiments performed in triplicate are shown. (B) Quantification of E-cadherin–mRuby2 expression in isolated mesenchymal cells. Fluorescence intensity at 72 hours after exposure was quantified by ImageJ software, and plotted as means ± SD, n = 3. Statistical significance was assessed using 1-way analysis of variance, Dunnett’s posttest; ∗∗P = .001 to .01; ∗∗∗P = .0001.