Figure 7.

Stx2a induces necrosis and apoptosis in epithelial cells. (A) Enteroids derived from a transplanted HIO were challenged with Stx2a by luminal or interstitial exposure, stained for necrosis (Sytox-green), and whole-mount tissues were stained for apoptosis (caspase 3, red). Scale bar: 100 μm. Representative images of experiments performed in triplicate. (B and C) Cellular death quantified by fluorescence using ImageJ software, and plotted as means ± SD, n = 3. Statistical significance compared with saline control was assessed using 1-way analysis of variance, Bonferroni posttest; ∗∗P = .001 to .01; ∗∗∗P < .0001. (B) Death by apoptosis (caspase 3). (C) Death by necrosis (Sytox).