Table 1.
Stx2a Expression Relative to Controls for Select Intestinal and Lineage-Specific Genes
| Symbol | Name | Cellular distribution or function | Stx2a 4 h fold change (P value adjusted)a | Stx2a 24 h fold change (P value adjusted)a |
|---|---|---|---|---|
| Epithelial/mesenchymal proteins | ||||
| CDH1 | Cadherin 1, type 1, E-cadherin | Epithelial Adherens junction |
2.4 (.03) | 0.94 (.95) |
| KLF4 | Kruppel-like factor 4 (gut) | Transcription factor, up-regulates E-cadherin | 5.9 (.00023) | 0.86 (.90) |
| CTNNB1 | Catenin β 1 (cadherin-associated protein) | Epithelial Adherens junction |
1.1 (.9) | 0.96 (.97) |
| LGALS4 | Lectin, galactoside-binding, soluble, 4 | Epithelial Adherens junction |
17 (6E-08) | 0.67 (.68) |
| TJP2 | Tight junction protein 2 (ZO-2) | Epithelial Tight junction |
2.0 (.03) | 0.91 (.90) |
| OCLN | Occludin | Epithelial Tight junction |
2.4 (.007) | 1.2 (.80) |
| CLDN1 | Claudin 1 | Epithelial Tight junction |
2.8 (.0006) | 0.74 (.73) |
| VIL1 | Villin 1 | Epithelial Brush border |
6.3 (.0005) | 0.82 (.80) |
| ALPI | Alkaline phosphatase, intestinal | Epithelial Enterocytes |
55 (.02) | 1.3 (.89) |
| LYZ | Lysozyme | Epithelial Paneth cells |
7.3 (.0001) | 0.64 (.52) |
| CHGA | Chromogranin A | Epithelial Enteroendocrine cells |
0.99 (1.0) | 0.37 (.26) |
| MUC2 | Mucin 2 oligomeric mucus/gel-forming | Epithelial Goblet cells |
11 (.0005) | 2.7 (.008) |
| MUC13 | Mucin 13, cell surface associated | Epithelial Mucus |
10 (8E-06) | 0.94 (.98) |
| MUC17 | Mucin 17, cell surface associated | Epithelial Mucus |
14 (.001) | 3.1 (.97) |
| TFF1 | Trefoil factor 1 | Epithelial Mucosal layer |
32 (3E-11) | 1.4 (.80) |
| TFF2 | Trefoil factor 2 | Epithelial Mucosal layer |
58 (4E-10) | 2.0 (.60) |
| TFF3 | Trefoil factor 3 (intestinal) | Epithelial Mucosal layer |
15 (5E-07) | 1.3 (.83) |
| VIM | Vimentin | Mesenchyme | 0.89 (.8) | 0.92 (.93) |
| Immune response | ||||
| IL18 | Interleukin 18 | Immune response | 12 (6E-08) | 0.40 (.41) |
| CCL15 | Chemokine (C-C motif) ligand 15 | Immune response | 32 (1E-08) | 0.9 (.95) |
| Shiga toxin receptor, proliferation, apoptosis | ||||
| A4GALT | Gb3 synthase | Shiga toxin receptor | 0.10 (.3) | 1.9 (.86) |
| EGFR | Epidermal growth factor receptor | Cellular growth | 0.37 (9E-06) | 2.1 (.02) |
| MKI67 | Marker of proliferation Ki-67 | Cellular growth | 0.30 (2E-08) | 1.7 (.50) |
| XBP1 | X-box binding protein 1 | ER stress | 1.5 (.19) | 0.69 (.53) |
| DNAJB9 | DnaJ (Hsp40) homolog, subfamily B, member 9 | ER stress | 1.3 (.53) | 1.1 (.97) |
| DDIT3 | CHOP, DNA-damage-inducible transcript 3 | ER stress | 1.5 (.60) | 1.1 (.94) |
| BCL2 | B-cell CLL/lymphoma 2 | Anti-apoptotic | 0.32 (.17) | 0.69 (.79) |
| TNFRSF10B | DR5, tumor necrosis factor receptor superfamily, member 10b | Pro-apoptotic | 0.97 (.96) | 0.89 (.88) |
| NLRP3 | NLR family, CARD domain–containing 4 | Interacts with the apoptosis-associated speck-like protein PYCARD | 0.55 (.88) | 0.52 (.76) |
| PYCARD | PYD and CARD domain containing | Promotes caspase-mediated apoptosis | 2.2 (.43) | 0.61 (.68) |
| CSP4 | Caspase-4 | Inflammatory caspase, promotes pyroptosis through gasdermin D cleavage | 2.3 (.04) | 0.83 (.87) |
ER, endoplasmic reticulum.
a
Adjusted for false-discovery rate.