Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 14;9(5):bio052027. doi: 10.1242/bio.052027

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 7. — Urp over-expression causes dorsal curvature of the body axis in zebrafish embryos. (A) Wild-type embryos injected with hs::urp2 transgene, without heat induction. Note no effect on the body axis. (B) Same batch of embryos imaged after 30 min post-heat shock. Note the initiation of dorsal curvature of the body axis in the heat-shocked embryos (arrows). (C) A wild-type embryo at 72 hpf injected with myog::urp2 transgene, showing dorsal curvature of the trunk and tail (arrow). (D) A wild-type embryo at 5 dpf injected with myog::urp2 transgene showing spiral coiling of the body axis (arrow). Embryos shown in A–D are representative of a minimum of 100 embryos analysed for each condition of Urp2 over-expression, in two independent experiments. (E) A wild-type embryo at 48 hpf, injected with water in the dorsal somite, imaged immediately after injection. (F) The same wild-type embryo depicted in E, imaged after 1 h. Note no effect on body axis position. (G) A wild-type embryo at 48 hpf, injected with synthetic Urp1 in the dorsal somite, imaged immediately after injection. (H) The same wild-type embryo depicted in G, imaged after 5 min. Note the dorsal curvature of the body axis (arrow). In E and G, the injection sites are indicated (magenta arrows). Four embryos were injected for each condition. Scale bars: 1 mm.