Table 4.
Concordance between genotyping results of LAMP-MC method with the referent method.
| Gene /SNP | Sample type | Sample number | Interpreted melting curves | Valid result | False resulta | Concordance (%) | Cohen’s κ Coefficientb |
|---|---|---|---|---|---|---|---|
| ADORA2A / | Blood | 30 | 30 | 29 | 1 | 96.7 | 0.936 |
| rs5751876 | Bu Cells | 12 | 12 | 12 | 0 | 100 | 1 |
| TNF-α / | Blood | 30 | 29 | 28 | 1 | 96.6 | 0.910 |
| rs1800629 | Bu Cells | 12 | 12 | 11 | 1 | 91.7 | 0.824 |
| ADA / | Blood | 30 | 30 | 30 | 0 | 100 | 1 |
| rs73598374 | Bu Cells | 12 | 12 | 12 | 0 | 100 | 1 |
| COMT / | Blood | 30 | 29 | 25 | 4 | 86.2 | 0.610 |
| rs4680 | Bu Cells | 12 | 0 | – | – | – | – |
| PER3 / | Blood | 30 | 28 | 27 | 1 | 96.4 | 0.870 |
| rs228697 | Bu Cells | 12 | 12 | 12 | 0 | 100 | 1 |
Bu Cells means Buccal cells.
a
Heterozygous not detected (one of the two peaks too weak).
b
Cohen’s kappa values are ranked as minimal agreement (κ<0.20), weak agreement (κ<0.40), moderate agreement (κ<0.60), good agreement (κ<0.80) and excellent agreement (κ≥0.80).