Table 1. Theoretical and Experimental MWs of Recombinant DcpS Proteins.
| theoretical MW (Da) | experimental MW (Da) | ||
|---|---|---|---|
| human DcpS | N-tagged | 41129.46 | 41164.50 |
| 40987.00 | |||
| (Δ 177.4) | |||
| nativea | 38745.94 | 38741.50 | |
| C-tagged | 39673.92 | 39532.00 | |
| (−141.9) | |||
| C. elegans DcpS | N-tagged | 38997.17 | 39042.50 |
| 38865.50 | |||
| (Δ 177.0) | |||
| nativea | 36613.65 | 36609.50 | |
| C-tagged | 38210.42 | 38204.00 | |
| A. suum DcpS | N-tagged | 37494.56 | 37363.00 |
| 37541.00 | |||
| (Δ 178.0) | |||
| nativea | 35111.04 | 35109.50 | |
| C-tagged | 36252.25 | 36113.50 | |
| (−138.75) |
a
MW of the native forms of DcpS containing one additional histidine as a result of His-tag cleavage at their N-terminus.
In brackets, the difference between two ESI-MS peaks for N-tagged proteins (Δ177 Da) or between theoretical and experimental MWs for C-tagged hDcpS and AsDcpS is indicated.