Table 2. Melting Temperature Values (T_m) of Studied DcpS Proteins in the apo Form and in the Presence of their Ligand m⁷GDP Determined by DSF^a.

	H. sapiens DcpS		C. elegans DcpS		A. suum DcpS
	Tm, °C apo	Tm, °C+m7GDP	Tm, °C apo	Tm, °C+m7GDP	Tm, °C apo	Tm, °C+m7GDP
WT(untagged)	58.2 ± 0.2	67.0 ± 0.1	47.9 ± 0.7	56.8 ± 0.3	61.8 ± 0.2	74.8
	N = 3	N = 2	N = 4	N = 4	N = 2	N = 1
N-tagged	59.5 ± 0.5	68.0	36.9 ± 0.1 and51.0 ± 0.2	55.7 ± 0.6	62.3 ± 0.4	75.8 ± 0.3
	N = 2	N = 1	N = 7	N = 4	N = 6	N = 2
C-tagged	59.5 ± 0.5	67.0	42.3 ± 0.3	56.6 ± 0.3	59.3 ± 0.2	75.0
	N = 4	N = 1	N = 4	N = 3	N = 4	N = 1

^aCalculated T_m values, determined as the minima of the first negative derivative of the melting curve (Figure 2), correspond to the arithmetic mean of the indicated number (N) of independent experiments (± SE of the mean).