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. Author manuscript; available in PMC: 2020 May 21.
Published in final edited form as: Methods Mol Biol. 2019;2009:99–109. doi: 10.1007/978-1-4939-9532-5_8

Figure 1.

Figure 1

Schematic of mechanism of DPPs, which “turn-on” when processed by acyl protein thioesterases (APTs). A peptide substrate featuring an acylated cysteine residue is tethered to a pro-fluorescent molecule by a biologically stable carbamate linker. APT s activity on the peptide and removal of the thioester unmasks the thiol of the cysteine, which results in a cascade of rapid intramolecular cyclization, lactone opening, and release of a fluorescent product.