FIGURE 5.

Effect of the combination of palbociclib and AQB inhibitors on the cell cycle. A and B, The colony formation assays and CCK‐8 experiments demonstrate the effect of DMSO, AQB, palbociclib, and combination therapy on the cell proliferation in glioma cells. C, The cell cycle distribution of U87 and N33 was measured using flow cytometry. D and E, The qPCR and western blot data confirmed that the decrease of CDK4, CDK6, and p‐RB in glioma cells was more pronounced after the combined administration (^* P < .05, ^** P < .01, ^*** P < .001, ^**** P < .0001). F, The confocal microscopy images of F‐actin (filamentous pseudopodia formation of F‐actin) and CDK6 in N33 and U87 cells using AQB and palbociclib alone, or in combination. DAPI was used to stain the core. Bar, 20 mm