Fig. 4.

Immunohistochemical analysis of hepatic tumor. a Comparison of SOX10, S100 and HMB45 expression in the liver of splenic injection model and hepatic injection model. Liver tissues in each mouse model at 6 and 8 weeks after injection were stained with SOX10 (100x). Liver 6 weeks or 8 weeks after injection of tumor cells; b splenic injection with splenectomy, c splenic injection without splenectomy, d hepatic injection and e tail vein injection (100 × and 200x). f Lung in each model were stained with SOX10 (100x). Scale bar = 500 μm