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Implementation of an “HCD-only” DDA acquisition workflow. (A) MS/MS spectra of alanyltyrosine obtained upon HCD activation at 10%, 30% and 50%, and stepped HCD at 30% ± 20%. (B) Schematic overview of the “HCD-only” DDA method (More detailed information regarding experimental conditions and filters is given in Supplementary Material, Table S2).
