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. 2020 May 8;5(19):11241–11246. doi: 10.1021/acsomega.0c01588

Figure 4.

Figure 4

Effect of NMT on Dex-induced myotube atrophy in C2C12 cells. Myotubes differentiated from C2C12 myoblasts were treated with Dex or Dex + NMT (10 mM) for 24 h. (A) Representative images for control, Dex, and Dex + NMT groups (bars = 200 μm). (B) Frequency and (C) average values of myotube diameters were calculated from more than 100 myotubes. (C) Data are presented as means ± SD of measurements from more than 100 myotubes per group. Similar results were obtained from three independent experiments. *p < 0.001 vs control, $p < 0.001 vs Dex alone. (D) Gene expression of Atrogin-1 and MuRF1 in C2C12 myotubes treated with Dex and NMT for indicated time points. The expression level is normalized by the GAPDH mRNA level. Data are presented as means ± SD of four replicates in four independent experiments. *p < 0.05; **p < 0.01 vs control.