Table 1. LC Gradient Program for Chromatographic Separation of 8-Isoprostane^a.

time	module	event	% mobile phase B by volume
0.01	controller	solenoid valve
0.02	controller	start
0.06	autosampler	inject
0.30	pumps	%B	40
0.31	pumps	%B	55
7.00	pumps	%B	65
7.01	pumps	%B	100
9.00	pumps	%B	100
9.01	pumps	%B	40
11.00	controller	stop

^aMobile phase A is 0.15% formic acid in water and mobile phase B is acetonitrile/0.15% formic acid in water (v/v, 1:1)