Table 3.

Determination of C_q values obtained from the amplification of tenfold dilutions of a purified amplicon of the cps3K gene of the strain NCIMB 703043 obtained by qPCR

	Intra-assay variance			Inter-assay variance
DNA concentration (ng/μl)	Cq values	No. of target copies	%CV	Cq values	No. of target copies	%CV
3.8 × 10−3	6.77 ± 0.51	2.67 × 108	7.53	6.48 ± 0.41	2.67 × 108	6.33
3.8 × 10−4	10.40 ± 0.10	2.67 × 107	0.96	10.71 ± 0.44	2.67 × 107	4.09
3.8 × 10−5	14.43 ± 0.08	2.67 × 106	0.55	13.97 ± 0.65	2.67 × 106	4.66
3.8 × 10−6	17.11 ± 0.21	2.67 × 105	1.23	16.98 ± 0.19	2.67 × 105	1.12
3.8 × 10−7	19.83 ± 0.06	2.67 × 104	0.30	19.90 ± 0.10	2.67 × 104	0.50
3.8 × 10−8	24.07 ± 0.04	2.67 × 103	0.17	23.80 ± 0.39	2.67 × 103	1.63
3.8 × 10−9	27.21 ± 0.11	2.67 × 102	0.40	27.13 ± 0.12	2.67 × 102	0.44