Figure 2.

miR-30a-5p specifically targets SOX9. (a) The miRNAs regulating SOX9 predicted from DIANA, TargetScan, miRDB, miRpath and miRDIP. (b) Binding sites between miR-30a-5p and the 3′UTR of SOX9 mRNA and luciferase activity of SOX9-WT and SOX9-MUT after miR-30a-5p mimic transfection. (c) The mRNA expression of SOX9 after the overexpression of miR-30a-5p determined by RT-qPCR. (d) The protein expression of SOX9 normalized to GAPDH after overexpression or inhibition of miR-30a-5p measured by western blot analysis. *p < 0.05 versus the NC mimic or NC inhibitor group. Measurement data were expressed as mean ± standard deviation. Comparison between two groups was analysed by unpaired t-test. Data at different time points were compared by repeated-measures ANOVA, followed by Tukey's post hoc test. Cell experiment was repeated three times independently.