Table 2.

Primer sequences for RT-qPCR. RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-30a-5p, MicroRNA-139-5p; SOX9, SRY-box 9; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.

gene	primer sequence
miR-30a-5p	F: 5′-ATTGCTGTTTGAATGAGGCTTCAGTACTTT-3′ R: 5′-TTCAGCTTTGTAAAAATGTATCAAAGAGAT-3′
SOX9	F: 5′-TTTCCAAGACACAAACATGA-3′ R: 5′-AAAGTCCAGTTTCTCGTTGA-3′
U6	F: 5′-CTCGCTTCGGCAGCACA-3′ R: 5′-AACACTTCACGAATTTGCGT-3′
GAPDH	F: 5′-GGTGAAGGTCGGTGTGAACGGATTTGG-3′ R: 5′-TGTGCCGTTGAATTTGCCGTGAGTGG-3′