Table I.
Genes | RFLP-PCR primers (5'-3') (13,14)a | Reverse transcription-quantitative PCR primers (5'-3') (15)a |
---|---|---|
MMP3 | F: CTTCCTGGAATTCACATCACTGCCACCACT | F: GTCTCTTTCACTCAGCCAAC |
R: GGTTCTCCATTCCTTTGATGGGGGGAAAGA | R: ATCAGGATTTCTCCCCTCAG | |
MMP9 | F: GCCTGGCACATAGTAGGCCC | F: CCTTCCTTATCGCCGACAAG |
R: CTTCCTAGCCAGCCGGCATC | R: TGAACAGCAGCATCTTCCCC | |
GAPDH | - | F: GACCTGCCGTCTAGAAAAAC |
- | R: TTGAAGTCAGAGGAGACCAC |
aReferences for the primers used in RFLP-PCR and quantitative PCR. MMP, matrix metalloproteinase. RFLP-PCR, restriction fragment length polymorphism-PCR; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; F, forward; R, reverse.