a, MHC I expression in control (grey) and
B2m-null (purple) B16 tumour cell lines relative to isotype
control (dashed line). Data are representative of two independent experiments.
b, T cell killing assay with non-ovalbumin-expressing control
(dashed line), ovalbumin-expressing control (grey) and ovalbuminexpressing
B2m-null (purple) B16 cells (n = 3 for
effector target ratios 1:20, 1:10 and 1:5 for each condition; data shown are
representative of two independent experiments). c, Tumour volume
following anti-PD-1 and GVAX treatment of B16 tumours with the genetic
perturbations indicated. Data (mean ± s.e.m.) are representative of two
independent experiments with n = 5 mice in each group.
d, Flow cytometry of immune populations following anti-PD-1 and
GVAX treatment of B16 tumours with the genetic perturbations indicated
(n = 5 mice per group). Bars represent mean.
c, d, Two-sided Student’s
t-test; *P < 0.05; **P
< 0.01; ***P < 0.001; ****P
< 0.0001; NS, not significant.