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. Author manuscript; available in PMC: 2021 Jan 20.
Published in final edited form as: Circ Heart Fail. 2020 Jan 20;13(1):e006525. doi: 10.1161/CIRCHEARTFAILURE.119.006525

Figure 2. Downregulation of Ahit promotes PE-induced cardiomyocyte hypertrophy.

Figure 2.

A. Ahit expression in NRCMs transfected with siRNA targeting Ahit (siAhit) or control siRNA (scramble) for 24 h (n=4). B-D. Effect of PE (10 μM/48h) in NRCMs (NMCMs were not used because they do not hypertrophy in response to PE, data not shown) pretreated with siAhit (SiAhit+PE) or scramble siRNA (Scramble+PE). Another group of NRCMS were treated with scramble siRNA but not PE (scramble). Representative immunohistochemistry images of NRCMs stained with α-actinin in red and 4′,6- diamidino-2-phenylindole (DAPI) in blue. (B). Fold-change of the cell surface area of α-actinin-positive NRCMs (n=3 slides with 10 fields/slide, one-way ANOVA, Holm-Sidak test) (C). The mRNA expressions of hypertrophy marker genes ANP, BNP, and β-MHC in NRCMs were quantified by qRT-PCR (n=3, **P < 0.01 versus scramble or scramble+PE, one-way ANOVA, Holm-Sidak test) (D). Data are shown as mean±SEM.