A, B. Oil red O staining (A) and IHC of β-casein (B) show poor milk productions in Scribf/fPgrCre/+ mammary glands on lactation day 2. Scale bar: 50 μm (A) and 20 μm (B).
C. IF of Ki67 (cell proliferation marker) and CK8 (epithelial cell marker) indicates reduced proliferation of alveolar cells in Scribf/fPgrCre/+ mammary glands on lactation day 2. Scale bar: 50 μm.
Each image is a representative from at least 3 independent experiments.