REAGENTS OR RESOURCES	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli BL21(DE3)	Thermo Scientific	Cat#EC0014
pDENV-Luc infectious clone	Marceau et al. (2016)	N/A
pDENV-Luc replicon	Marceau et al. (2016)	N/A
Chemicals, Peptides and Recombinant Proteins
DMEM	Gibco	Cat#11995065
Trypsin/EDTA	Gibco	Cat#25300054
Penicillin-Streptomycin	Gibco	Cat#15140122
Glutamine	Gibco	Cat#25030081
Fetal Bovine Serum	HyClone	Cat#SH30396.03
Electroporation Buffer for replicon	Teknova	Cat#E0399
XbaI Restriction Enzyme	New England BioLabs	Cat#R0145S
Lactate dehydrogenase, porcine heart (LDH)	EMD Millipore	Cat#427211
Pyruvate kinase, rabbit muscle type III (PK)	Sigma-Aldrich	Cat#P9136
Adenosine triphosphate disodium trihydrate (ATP)	VMR Life Science	Cat#0220
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH)	Santa Cruz	Cat# sc-205762A
Phosphoenolpyruvate monopotassium salt (PEP)	Sigma-Aldrich	Cat#P7127
Cytidine 5’-monophospahte disodium salt (CMP)	Sigma-Aldrich	Cat#C1006
Uridine	Sigma-Aldrich	Cat#U3750
LB Broth	Fisher BioReagents	Cat#BP97235
Isopropyl-1-thio-b-D-galacto-pyranoside (IPTG)	Thermo Fisher Scientific	Cat#R0392
R1479	MedChemExpress	Cat#HY-10444
Dipyridamole (DPY)	MP Biomedicals	Cat#0215372205
D-ribose	Chem Impex	Cat#50494709
Tetrabutylammonium fluoride solution (TBAF), 1.0M	Sigma-Aldrich	Cat#216143
Diethyl azodicarboxylate solution (DEAD), 40 wt.%	Sigma-Aldrich	Cat#563110
Triphenylphosphine (PPh3)	Sigma-Aldrich	Cat#T84409
Uracil	Sigma-Aldrich	Cat#U0750
5-Fluorouracil	Sigma-Aldrich	Cat#F6627
Thymine	AK Scientific	Cat#J91030
5-Iodouracil	AK Scientific	Cat#K165
Ammonia, ca. 7N solution in methanol	Sigma-Aldrich	Cat#499145
Silver (I) oxide (Ag2O)	Sigma-Aldrich	Cat#226831
Methyl iodide (MeI)	EMD Millipore	Cat#IX0185
Diethylaminosulfur trifluoride (DAST)	Oakwood	Cat#002323
Methanesulfonyl chloride (MsCl)	Sigma-Aldrich	Cat#471259
Triethylamine (NEt3)	Thermo Fisher Scientific	Cat#04885
Acetyl chloride (AcCl)	Sigma-Aldrich	Cat#114189
Palladium hydroxide on carbon [Pd(OH)2/C], 5 wt.%	Acros Organic	Cat#199620100
Cyclohexene	Sigma-Aldrich	Cat#29240
Sodium azide (NaN3)	Sigma-Aldrich	Cat#71289
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES)	Fisher BioReagents	Cat#BP310-100
Tris(hydroxymethyl)aminomethane base (Tris)	Fisher BioReagents	Cat#BP152-1
Dithiothreitol (DTT)	Fisher BioReagents	Cat#BP172-25
Magnesium chloride (MgCl2)	Fisher Chemical	Cat#M33-500
Potassium chloride (KCl)	Fisher Chemical	Cat#P217-500
Medronic acid (InfinityLab Deactivator Additive)	Agilent	Cat#5191-3940
Adenosine 15N5 5’-monophosphate disodium salt	Sigma-Aldrich	Cat#662658
Uridine 15N2 5’-monophosphate sodium salt	Sigma-Aldrich	Cat#662666
Critical Commercial Assays
MEGAscript T7 Transcription Kit	Invitrogen	Cat#AM1334
m7G(5')ppp(5')G RNA Cap Structure Analog	New England BioLabs	Cat#S1404S
CellTiter-Glo	Promega	Cat#G7570
Renilla-Glo	Promega	Cat#E2710
Renilla	Promega	Cat#E2820
Experimental Models: Cell Lines
A549	ATCC	Cat#CCL-185
Vero E6	ATCC	Cat#CRL-1586
Recombinant DNA
pET21a-UCK2 expression plasmid	Okesli-Armlovich et al. (2019)	N/A
pET28-CMPK1 expression plasmid	Deans et al. (2016)	N/A
Software
Prism 8.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
ChemDraw Professional 17.1	PerkinElmer	https://www.perkinelmer.com/category/chemdraw
MNova 12.0	Mestrelab	https://mestrelab.com/download_file/mnova-12-0-0/
Other
Centrifugal Filter Unit	Amicon	Cat#UFC900308
Hi Trap Q anion exchange column	GE Healthcare Life Sciences	Cat#17115301