Figure S4.

Virus Isolation from Infected Mouse Lung and Brain Tissues, Related to Figure 3 and Table S3

Infected mouse lung and brain tissues were homogenized with DMEM, and the supernatant was used to infect Vero E6 cells. CPE was observed in cells infected by the homogenized supernatant of lung (B) and brain (C) but not in mock-infected cells (A). Viral antigen was detected in the cells infected by homogenized supernatant of the lung (E) and brain (F) but not in mock-infected cells (D). Scale bar: 1000 μm (A, B, C) and 200 μm (D, E, F).