REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-RP3-CoV N protein polyclonal antibody	Zhou et al., 2020	N/A
Cy3-conjugated goat-anti-rabbit IgG	Abcam	Cat#ab6939; RRID:AB_955021
DAPI	Beyotime	Cat#C1002
Bacterial and Virus Strains
SARS-CoV-2	Isolated from patient Zhou et al., 2020	IVCAS 6.7512
Chemicals, Peptides, and Recombinant Proteins
Avertin	Sigma	Cat#T-48402-5g
Hematoxylin solution	Servicebio	Cat#G1005-1
Eosin solution	Servicebio	Cat#G1005-2
IDEXX Catalyst BUN	IDEXX	Cat#98-11072-01
IDEXX Catalyst CK	IDEXX	Cat#98-11073-01
IDEXX Catalyst AST	IDEXX	Cat#98-11069-01
IDEXX Catalyst ALT	IDEXX	Cat#98-11067-01
4% paraformaldehyde	Boster	Cas#AR1068
RNAlater Stabilization Solution	Invitrogen	Cat#AM7021
Triton X-100	Sigma	Cas#9002-93-1
EDTA-Na2	Sigma	Cas#6381-92-6
Critical Commercial Assays
QIAamp 96 Virus QIAcube HT Kit	QIAGEN	Cat#57731
HiSxript II One step qRT-PCR SYBR Green Kit	Vazyme	Cat#Q221-01
Modified MSB staining Kit	Solarbio	Cat#G2040
IDEXX ProCyte Dx Reagent Kit	IDEXX	Cat#99-26306-00
Experimental Models: Cell Lines
Vero E6	ATCC	ATCC CRL-1586; RRID:CVCL_0574
HFH4-hACE2 mice	Ralph S. Baric’s lab	N/A
Oligonucleotides
RBD-qF1: 5′- CAATGGTTTAACAGGCACAGG-3′	Zhou et al., 2020	N/A
RBD-qR1: 5′- CTCAAGTGTCTGTGGATCACG-3′	Zhou et al., 2020	N/A
Software and Algorithms
Pannoramic Viewer	3DHISTECH	https://www.3dhistech.com/software-downloads/
StepOne Software	ABI	N/A
GraphPad Prism 8	GraphPad Software	https://www.graphpad.com
Geneious	Geneious 10.2.6	https://www.geneious.com