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. Author manuscript; available in PMC: 2020 May 21.
Published in final edited form as: J Vis Exp. 2020 Feb 27;(156):10.3791/60792. doi: 10.3791/60792

Figure 3: Time-lapse imaging of dendrite degeneration and exposure of an eat-me signal.

Figure 3:

Selected frames from a time-lapse movie of degenerating dendrites of a class IV da neuron after laser injury. Dendrites were labeled by ppk>CD4-tdTom. The eat-me signal PS on degenerating dendrites was detected by Annexin-GFP (AV-GFP), which is secreted by the fat body. Yellow arrows point to the branches showing AV-GFP labeling. Blue arrows point to dendrites undergoing fragmentation.