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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Nat Metab. 2020 May 21;2(5):397–412. doi: 10.1038/s42255-020-0205-x

Figure 8: Phylogenetic analysis and conceptual function model for LINC00473.

Figure 8:

a.We used BLASTN 2.8.0+ against “nr” database to find orthologs of the NR_026860.1 (Homo sapiens long intergenic non-protein coding RNA 473 (LINC00473), transcript variant 1, long non-coding RNA molecule type nucleic acid) in other organisms. To be as inclusive as possible we used the “More dissimilar sequences” (discontiguous megablast) option. We detected 243 homolog sequences from the blast. The pairwise sequence distances were used to generate a phylogenetic tree using fast minimum evolution tree method with 0.65 cut-off for maximum sequence differences. Although we did find some homolog sequences in primates, we did not detect any significant homolog sequence in lower eukaryotes. b. Conceptual model in which LINC00473 expression regulates inter-organelle communication upon activation. Activation of thermogenic adipocytes leads to cAMP-dependent expression and cytoplasmic translocation of LINC00473 to the lipid droplet-mitochondria interface, where it forms multimeric complexes that include PLIN1. Through these interactions, lipolysis and mitochondrial respiration are activated. A feedback loop where PLIN1 levels influence LINC00473 expression contributes to this regulatory mechanism.